Single-round isolation of diverse RNA aptamers from a random sequence pool
نویسندگان
چکیده
منابع مشابه
Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Riboswitches are shape-changing regulatory RNAs that bind chemicals and regulate gene expression, directly coupling sensing to cellular actuation. However, it remains unclear how their sequence controls the physics of riboswitch switching and activation, particularly when changing the ligand-binding aptamer domain. We report the development of a statistical thermodynamic model that predicts the...
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ژورنال
عنوان ژورنال: Biology Methods and Protocols
سال: 2018
ISSN: 2396-8923
DOI: 10.1093/biomethods/bpy004